Journal article

Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres

Faten Zaibak, Paul Bello, Jennifer Kozlovski, Duncan Crombie, Haozhi Ang, Mirella Dottori, Robert Williamson

BMC BIOTECHNOLOGY | BMC | Published : 2009


BACKGROUND: Human umbilical cord blood-derived unrestricted somatic stem cells (USSCs), which are capable of multilineage differentiation, are currently under investigation for a number of therapeutic applications. A major obstacle to their clinical use is the fact that in vitro expansion is still dependent upon fetal calf serum, which could be a source of pathogens. In this study, we investigate the capacity of three different stem cell culture media to support USSCs in serum-free conditions; HEScGRO, PSM and USSC growth medium ACF. Our findings demonstrate that USSCs do not grow in HEScGRO or PSM, but we were able to isolate, proliferate and maintain multipotency of three USSC lines in USS..

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Funding Acknowledgements

We thank the New Zealand Cystic Fibrosis Association, Australian Cystic Fibrosis Research Trust, University of Melbourne, and a group of generous donors that initiated the establishment of this research program. Special thanks to: Professor Gesine Kogler, who advised us on the creation of the USSC line; Jacqui Johnson and Jenny Greenhalgh for reagent support; Dr Alice Pebay for providing PSM; and Pei Tian and Dr Caitlin Filby for their technical support.