Journal article

Formation of 5 alpha-reduced androgens in the testes and urogenital tract of the grey short-tailed opossum, Monodelphis domestica

Jean D Wilson, Marilyn B Renfree, Richard J Auchus, Andrew J Pask, Geoffrey Shaw



Testicular 5alpha-reduced androgens, largely 5alpha-androstane-3alpha,17beta-diol (androstanediol), are responsible for virilisation of pouch young in one marsupial (the tammar wallaby), but are not formed until later in development in another marsupial (the brushtail possum) and in rodents. Because the mechanism of virilisation of the urogenital tract in the grey short-tailed opossum Monodelphis domestica has never been defined, androgen formation and metabolism were investigated in this species. Testis fragments from grey short-tailed opossums of a wide range of ages were incubated with [3H]-progesterone and the metabolites were separated by high-performance liquid chromatography (HPLC). T..

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Awarded by National Health and Medical Research Council of Australia (NHMRC)

Awarded by Robert A. Welsh Foundation

Funding Acknowledgements

This study was supported by grant 208911 from the National Health and Medical Research Council of Australia (NHMRC) and by Grant I-1493 from the Robert A. Welsh Foundation. A.J.P. was supported by an NHMRC RD Wright Fellowship and M. B. R. was supported by an Australian Research Council Federation Fellowship. The authors thank C. David Vance for performing the HPLC of the samples and Professor Norman Saunders and Dr Joakim Ek for providing the opossums.