Journal article
Comparative analysis of the mammalian WNT4 promoter
H Yu, AJ Pask, G Shaw, MB Renfree
BMC Genomics | BMC | Published : 2009
Abstract
Background: WNT4 is a critical signalling molecule in embryogenesis and homeostasis, but the elements that control its transcriptional regulation are largely unknown. This study uses comparative cross species sequence and functional analyses between humans and a marsupial (the tammar wallaby,Macropus eugenii) to refine the mammalian Wnt4 promoter. Results: We have defined a highly conserved 89 bp minimal promoter region in human WNT4 by comparative analysis with the tammar wallaby. There are many conserved transcription factor binding sites in the proximal promoter region, including SP1, MyoD, NFκB and AP2, as well as highly conserved CpG islands within the human, mouse and marsupial promote..
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Funding Acknowledgements
We thank Susan Osborn for the tammar primary cell lines and Giovanna D'Abaco (Department of surgery, the University of Melbourne) for HEK293T cells. We also thank Natasha Sankovic and Amber Alsop for advice on the fluorescence in situ hybridization (FISH). This work was supported by the Australian Research Council Centre for Kangaroo Genomics, a Federation Fellowship to MBR and a National Health and Medical Research Council RD Wright Fellowship to AJP.