gamma delta TCR cells of the pregnant ovine uterus express variable T cell receptors and contain granulysin
Annette Fox, Jill F Maddox, Mike J de Veer, Els N Meeusen
JOURNAL OF REPRODUCTIVE IMMUNOLOGY | ELSEVIER IRELAND LTD | Published : 2010
Gammadelta T cells are a prominent granulated cell population in the ruminant pregnant uterus and both their number and granule size increase dramatically during pregnancy. Anchor-RT-PCR was used to assess TCRdelta gene usage by gammadelta T cells from the uterine epithelium of pregnant sheep. The TCRdelta genes obtained exhibited distinct combinatorial and junctional diversity and only two out of nine V-D-J rearrangements sequenced were identical. Furthermore, two of the Vdelta elements used are also expressed in peripheral blood, indicating that gammadeltaTCR use in sheep epithelia is neither restricted nor site-specific, similar to humans but in contrast to findings in mice. Protein analy..View full abstract
AF was supported by a Dora Lush, National Health and Medical Research Council postgraduate fellowship.