In response to oxidative stress, the expression of inflammatory cytokines and antioxidant enzymes are impaired in placenta, but not adipose tissue, of women with gestational diabetes
Martha Lappas, Amberlee Mittion, Michael Permezel
JOURNAL OF ENDOCRINOLOGY | BIOSCIENTIFICA LTD | Published : 2010
In response to oxidative stress, gestational diabetes mellitus (GDM) placenta releases less 8-isoprostane and tumour necrosis factor (TNF) alpha. The effect of oxidative stress on other cytokines and antioxidant gene expressions are unknown. The aim of this study is to further explore the antioxidant status and effect of oxidative stress in GDM tissue. Human placenta, omental and subcutaneous adipose tissue from women with and without GDM were exposed to hypoxanthine (HX)/xanthine oxidase (XO). Cytokine release was analysed by ELISA and cytokine and antioxidant gene expression by RT-PCR. Catalase (CAT) and glutathione reductase (GSR) mRNA expression was higher in GDM (n=18) compared with nor..View full abstract
Awarded by National Health and Medical Research Council
ML was a recipient of a National Health and Medical Research Council RD Wright Fellowship (grant no. 454777). The work described in this manuscript was funded by the National Health and Medical Research Council (grant no. 454310), Diabetes Australia Research Trust (DART), and the Medical Research Foundation for Women and Babies.