Cloning, purification and characterisation of brassinin glucosyltransferase, a phytoalexin-detoxifying enzyme from the plant pathogen Sclerotinia sclerotiorum
Adrienne C Sexton, Zoran Minic, Anton J Cozijnsen, M Soledade C Pedras, Barbara J Howlett
FUNGAL GENETICS AND BIOLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2009
The plant-pathogenic fungus Sclerotinia sclerotiorum can detoxify cruciferous phytoalexins such as brassinin via glucosylation. Here we describe a multifaceted approach including genome mining, transcriptional induction, phytoalexin quantification, protein expression and enzyme purification that led to identification of a S. sclerotiorum glucosyltransferase that detoxifies brassinin. Transcription of this gene, denoted as brassinin glucosyltransferase 1 (SsBGT1), was induced significantly in response to the cruciferous phytoalexins camalexin, cyclobrassinin, brassilexin, brassinin and 3-phenylindole, a camalexin analogue. This gene was also up-regulated during infection of Brassica napus lea..View full abstract
We thank the Grains Research and Development Corporation, Australia, the Canada Research Chairs program and the Government of Saskatchewan, Canada for funding. We are grateful to the B. cinerea and Sclerotinia sclerotiorum Sequencing Projects, Broad Institute of Harvard and MIT (http://www.broad.mit.edu) ' and The Research Unit in Genomics and Bioinformatics (URGI) (http://urgi.versailles.inra.fr/pascodb/botysclero/) and CA.Zy for access to the S. sclerotiorurn genome sequence and annotations.