Journal article

Inhibition of Matrix Metalloproteinase Activity in the Chick Sclera and Its Effect on Myopia Development

Hsin-Hua Liu, Alex Gentle, Andrew I Jobling, Neville A McBrien

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2010

Abstract

PURPOSE: To investigate the contribution of matrix degradation in the two-layer avian sclera to the development of myopia. METHODS: Tissue inhibitor of metalloproteinase-2 (TIMP-2) was used to inhibit chick scleral collagen degradation with (3)H-proline, a marker for this effect. Ex vivo scleral culture experiments confirmed TIMP-2 doses for in vivo experimentation. Ocular growth and refractive response to exogenous TIMP-2 (11.25, 2.25, and 0.45 picomoles, plus vehicle only) were monitored in 7-day-old chicks during the induction of myopia over 4 days with a translucent occluder. Collagen degradation was assessed, in whole sclera and in separated scleral layers by using the same paradigm (11..

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University of Melbourne Researchers