Inhibition of Matrix Metalloproteinase Activity in the Chick Sclera and Its Effect on Myopia Development
Hsin-Hua Liu, Alex Gentle, Andrew I Jobling, Neville A McBrien
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2010
PURPOSE: To investigate the contribution of matrix degradation in the two-layer avian sclera to the development of myopia. METHODS: Tissue inhibitor of metalloproteinase-2 (TIMP-2) was used to inhibit chick scleral collagen degradation with (3)H-proline, a marker for this effect. Ex vivo scleral culture experiments confirmed TIMP-2 doses for in vivo experimentation. Ocular growth and refractive response to exogenous TIMP-2 (11.25, 2.25, and 0.45 picomoles, plus vehicle only) were monitored in 7-day-old chicks during the induction of myopia over 4 days with a translucent occluder. Collagen degradation was assessed, in whole sclera and in separated scleral layers by using the same paradigm (11..View full abstract
Awarded by National Health and Medical Research Council of Australia
Supported by Project Grant 454602 from the National Health and Medical Research Council of Australia, and by a pilot grant from The University of Melbourne.