Journal article

Localization of matrix metalloproteinases (MMPs-2, 8, 9 and 20) in normal and carious dentine

Y Shimada, S Ichinose, A Sadr, MF Burrow, J Tagami

AUSTRALIAN DENTAL JOURNAL | WILEY | Published : 2009

Abstract

BACKGROUND: Dentine matrix metalloproteinases (MMPs) may participate in the destruction of dentine following demineralization by bacterial acids. This study investigated the localization of MMPs in carious dentine. METHODS: Frozen sections of dentine caries were prepared without demineralization and immersed in monoclonal antibody against MMP-2, -8, -9 and -20. The sections were labelled by IgG conjugated with gold colloidal particles, and observed under FE-SEM. Labelling indexes (number of gold particles/mum(2)) of outer and inner carious dentine, respectively, with and without bacterial infection, were compared with that of normal dentine. RESULTS: MMP-2 was distributed in both carious and..

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University of Melbourne Researchers