Localization of matrix metalloproteinases (MMPs-2, 8, 9 and 20) in normal and carious dentine
Y Shimada, S Ichinose, A Sadr, MF Burrow, J Tagami
AUSTRALIAN DENTAL JOURNAL | WILEY | Published : 2009
BACKGROUND: Dentine matrix metalloproteinases (MMPs) may participate in the destruction of dentine following demineralization by bacterial acids. This study investigated the localization of MMPs in carious dentine. METHODS: Frozen sections of dentine caries were prepared without demineralization and immersed in monoclonal antibody against MMP-2, -8, -9 and -20. The sections were labelled by IgG conjugated with gold colloidal particles, and observed under FE-SEM. Labelling indexes (number of gold particles/mum(2)) of outer and inner carious dentine, respectively, with and without bacterial infection, were compared with that of normal dentine. RESULTS: MMP-2 was distributed in both carious and..View full abstract
Awarded by Japan Society for the Promotion of Science
This study was financially supported by grants from the Japan Society for the Promotion of Science (No. 18592084).