Expression, Localization, and Function of the Thioredoxin System in Diabetic Nephropathy
Andrew Advani, Richard E Gilbert, Kerri Thai, Renae M Gow, Robyn G Langham, Alison J Cox, Kim A Connelly, Yuan Zhang, Andrew M Herzenberg, Per Knud Christensen, Carol A Pollock, Weier Qi, Sih Min Tan, Hans-Henrik Parving, Darren J Kelly
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY | AMER SOC NEPHROLOGY | Published : 2009
Excessive reactive oxygen species play a key role in the pathogenesis of diabetic nephropathy, but to what extent these result from increased generation, impaired antioxidant systems, or both is incompletely understood. Here, we report the expression, localization, and activity of the antioxidant thioredoxin and its endogenous inhibitor thioredoxin interacting protein (TxnIP) in vivo and in vitro. In normal human and rat kidneys, expression of TxnIP mRNA and protein was most abundant in the glomeruli and distal nephron (distal convoluted tubule and collecting ducts). In contrast, thioredoxin mRNA and protein localized to the renal cortex, particularly within the proximal tubules and to a les..View full abstract
Awarded by National Health and Medical Research Council Neil Hamilton Fairley scholarship
We thank M. Pacheco, J. Court, L DiRago, S. Glowacka, and S. Advani for excellent technical assistance. K.A.C. was Supported by a TACTICS scholarship (Canada), a National Health and Medical Research Council Neil Hamilton Fairley scholarship (440712), and a RACP Pfizer overseas grant.This work was presented in abstract form at the annual Meeting Of the American Society of Nephrology, October 31-November 5, 2007.