How does the Bax-alpha 1 targeting sequence interact with mitochondrial membranes? The role of cardiolipin
Marc-Antoine Sani, Erick J Dufourc, Gerhard Groebner
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | ELSEVIER | Published : 2009
A key event in programmed cell death is the translocation of the apoptotic Bax protein from the cytosol towards mitochondria. The first helix localized at the N-terminus of Bax (Bax-alpha1) can act here as an addressing sequence, which directs activated Bax towards the mitochondrial surface. Solid state NMR (nuclear magnetic resonance), CD (circular dichroism) and ATR (attenuated total reflection) spectroscopy were used to elucidate this recognition process of a mitochondrial membrane system by Bax-alpha1. Two potential target membranes were studied, with the outer mitochondrial membrane (OM) mimicked by neutral phospholipids, while mitochondrial contact sites (CS) contained additional anion..View full abstract
We are grateful to Axelle Grelard, Bernard Desbat, (Universite Bordeaux 1-ENITAB, France) and Goran Lindblom, Lennart Johansson and Eric Rosenbaum (Umea University) for all their support. This work was supported by Knut and Alice Wallenberg Foundation, Carl Kempe Foundation, Swedish Research Council, Umea University Insamlings-stiftelse and the Centre National de la Recherche Scientifique (CNRS). The Aquitaine region is acknowledged for equipment funding and the universities of Bordeaux 1 and Umea for setting up a co-tutoring Ph.D. program.