Journal article
Sertoli cell androgen receptor DNA binding domain is essential for the completion of spermatogenesis
P Lim, M Robson, J Spaliviero, KJ McTavish, M Jimenez, JD Zajac, DJ Handelsman, CM Allan
Endocrinology | Published : 2009
DOI: 10.1210/en.2009-0416
Abstract
We examined the biological importance of Sertoli cell androgen receptor (AR) genomic interaction, using a Cre-loxP approach to selectively disrupt theARDNA-binding domain (AR-DBD). Sertoli cell (SC)-specific transgenic Abpa or AMH promoters targeted Cre-mediated inframe excision of mouse Ar exon-3, encoding the AR-DBD second zinc-finger (ZF2), generating SC-specific mutant. ARΔZF2 lines designated Abp.SCARΔZF2 and AMH.SCARΔZF2, respectively. Both SCARΔZF2 lines produced infertile males exhibiting spermatogenic arrest, despite normal SC numbers and immunolocalized SC nuclear AR. Adult homozygous TgCre (+/+) SCARΔZF2 or double-TgCre(+/-) Abp/ AMH.SCARΔZF2 males displayed equivalent small teste..
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Funding Acknowledgements
We thank Mamdouh Khalil for excellent technical assistance with the animals.Address all correspondence and requests for reprints to: Charles M. Allan, ANZAC Research Institute, Concord Hospital, Sydney, New South Wales 2139, Australia. E-mail: charles@med.usyd.edu.au.This work was supported by the National Health and Medical Research Council Project and Australian Research Council Discovery Project grant funds.Disclosure Summary: The authors have nothing to disclose.