Purification of polyclonal anti-conformational antibodies for use in affinity selection from random peptide phage display libraries: A study using the hydatid vaccine EG95
AJ Read, CG Gauci, MW Lightowlers
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | ELSEVIER | Published : 2009
The use of polyclonal antibodies to screen random peptide phage display libraries often results in the recognition of a large number of peptides that mimic linear epitopes on various proteins. There appears to be a bias in the use of this technology toward the selection of peptides that mimic linear epitopes. In many circumstances the correct folding of a protein immunogen is required for conferring protection. The use of random peptide phage display libraries to identify peptide mimics of conformational epitopes in these cases requires a strategy for overcoming this bias. Conformational epitopes on the hydatid vaccine EG95 have been shown to result in protective immunity in sheep, whereas l..View full abstract
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Awarded by National Health and Medical Research Council
Awarded by Wellcome Trust
Funding is acknowledged from National Health and Medical Research Council grant 350279 and The Wellcome Trust grant 075818. The 20 mer library was kindly donated by AdAlta Pty Ltd., Department of Biochemistry, La Trobe University, Victoria 3086, Australia.