Journal article

Colony Stimulating Factor-1 Dependence of Paneth Cell Development in the Mouse Small Intestine

Duy Huynh, Xu-Ming Dai, Sayan Nandi, Sally Lightowler, Melanie Trivett, Chee-Kai Chan, Ivan Bertoncello, Robert G Ramsay, E Richard Stanley

GASTROENTEROLOGY | W B SAUNDERS CO-ELSEVIER INC | Published : 2009

Abstract

BACKGROUND & AIMS: Paneth cells (PCs) secrete defensins and antimicrobial enzymes that contribute to innate immunity against pathogen infections within the mucosa of the small intestine. We examined the role of colony stimulating factor-1 (CSF-1) in PC development. METHODS: CSF-1-deficient and CSF-1 receptor (CSF-1R)-deficient mice and administration of neutralizing anti-CSF-1R antibody were used to study the requirement of CSF-1 for the development of epithelial cells of the small intestine. CSF-1 transgenic reporter mice and mice that express only the membrane-spanning, cell-surface CSF-1 isoform were used to investigate regulation by systemic versus local CSF-1. RESULTS: Mice deficient in..

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