Journal article

Molecular Markers of Preterm Labor in the Choriodecidua

Renu Shankar, Matthew P Johnson, Nicholas A Williamson, Fiona Cullinane, Anthony W Purcell, Eric K Moses, Shaun P Brennecke

Reproductive Sciences | SAGE PUBLICATIONS INC | Published : 2010

Abstract

Because relevant biochemical changes are known to begin at the choriodecidual interface some weeks before actual clinical onset of labor, we hypothesized that the preterm choriodecidua may display gene and protein expression patterns specific to preterm labor. Transcriptomic (microarray) and proteomic (2-dimensional gel electrophoresis [2DGE]) profiling methodologies were used to compare changes in choriodecidual tissue collected from women who delivered before 35 weeks of gestation following spontaneous preterm labor (n = 12) and gestation-matched nonlaboring controls (n = 7). Additionally, 2DGE was used to compare differences in protein expression during term and preterm labor and to const..

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Grants

Awarded by National Institutes of Health


Awarded by National Center for Research Resources


Awarded by EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT


Awarded by NATIONAL CENTER FOR RESEARCH RESOURCES


Awarded by NATIONAL HEART, LUNG, AND BLOOD INSTITUTE


Awarded by NATIONAL INSTITUTE OF MENTAL HEALTH


Funding Acknowledgements

The study was conducted at The Royal Women's Hospital, Melbourne, Australia. This work was supported by scholarships and research funding from the University of Melbourne as well the Royal Women's Hospital, Melbourne, to RS and FC. Transcriptional profiling was supported by the National Institutes of Health (HD049847 to EKM) and conducted in facilities constructed with support from a Research Facilities Improvement Program grant C06 RR017515 from the National Center for Research Resources. The Ingenuity Pathway Analysis software license is supported by National Institutes of Health grants MH059490, HL045522, and HL028972. MPJ is the recipient of a Cowles Postdoctoral Fellowship. We thank Clinical Research Assistant Dr Alfia Al-Ghafra and Clinical Research Midwives Ms Sue Nisbet and Ms Sue Duggan for assistance with collecting the samples used in this study.