Journal article
Stimulation of the interleukin-1 receptor and Toll-like receptor 2 inhibits hepatitis B virus replication in hepatoma cell lines in vitro
AJ Thompson, D Colledge, S Rodgers, R Wilson, P Revill, P Desmond, A Mansell, K Visvanathan, S Locarnini
Antiviral Therapy | INT MEDICAL PRESS LTD | Published : 2009
DOI: 10.3851/IMP1294
Abstract
Background: Toll-like receptors (TLRs) are a key component of the innate immune system and TLR2 has been shown to be involved in the immunopathogenesis of hepatitis B virus (HBV) infection in vivo. We investigated the role of TLR2 stimulation of virus-infected hepatocyte cell lines as a potential antiviral mechanism in vitro. Methods: The hepatoblastoma cell line HepG2 was transduced with recombinant HBV baculoviruses and the hepatoma cell line Huh-7 was transiently transfected with complimentary DNA clones of HBV. HBV viral replication was quantified after stimulation with interleukin (IL)-1β and Pam-2-Cys, a synthetic TLR2 ligand, by measuring intracellular core-associated single-stranded ..
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Awarded by National Health and Medical Research Council of Australia (NHMRC)
Funding Acknowledgements
This work was supported in part from a National Health and Medical Research Council of Australia (NHMRC) postgraduate scholarship (Alex Thompson), the NHMRC Grant number 49101.7 and the Bristol-Myers Squibb Freedom To Discover Award to SL.