Murine hematopoietic blast colony-forming cells and their progeny have distinctive membrane marker profiles
Donald Metcalf, Ashley P Ng, Stephen JL Loughran, Belinda Phipson, Gordon K Smyth, Ladina Di Rago, Sandra Mifsud
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | NATL ACAD SCIENCES | Published : 2009
Two distinct bone marrow-derived blast colony-forming cells can generate colonies of lineage-restricted progenitor cells in agar cultures of murine bone marrow. Both cell types selectively had a Kit(+) ScaI(+) phenotype distinguishing them from most lineage-restricted progenitor cells. Multicentric blast colony-forming cells stimulated by stem cell factor plus interleukin-6 (IL-6) (BL-CFC-S) were separable from most dispersed blast colony-forming cells stimulated by Flt3 ligand and IL-6 (BL-CFC-F) using CD34 and Flt3R probes. Multicentric BL-CFC-S cofractionated with colony-forming units, spleen (CFU-S) supporting the possibility that the 2 cells may be identical. The colony populations gene..View full abstract
Awarded by National Health and Medical Research Council, Canberra
Awarded by National Institutes of Health, Bethesda
Awarded by NATIONAL CANCER INSTITUTE
This work was supported by the Carden Fellowship Fund of the Cancer Council, Victoria, the National Health and Medical Research Council, Canberra (Program 461219) and the National Institutes of Health, Bethesda (Grant CA22556). A.N. and S.J.L. were recipients of Australian Postgraduate Awards.