Journal article
Transcriptome analysis of in vivo and in vitro matured bovine MII oocytes
MG Katz-Jaffe, BR McCallie, KA Preis, J Filipovits, DK Gardner
Theriogenology | ELSEVIER SCIENCE INC | Published : 2009
Abstract
In vitro maturation (IVM) of mammalian oocytes does not support the same rates of embryo development or pregnancy when compared to oocytes that have matured in vivo. Therefore, environment has a significant influence on the oocyte's ability to complete maturation and acquire the mRNA and proteins required for successful fertilization and normal embryonic development. The aim of this study was to analyze the MII oocyte transcriptome between in vivo and in vitro conditions. Total RNA was extracted, processed and hybridized to the Affymetrix GeneChip® Bovine Genome Array. Following normalization of the microarray data, analysis revealed 10 differentially expressed genes after IVM compared to in..
View full abstractGrants
Funding Acknowledgements
The authors thank Prof. George Seidel Jr., D.J. Walker, and the animal reproduction group at Colorado State University, for their assistance with bovine oocyte collection. This work was partly funded by an independent grant from the Serono Research Institute.