Phosphorylation regulates copper-responsive trafficking of the Menkes copper transporting P-type ATPase

NA Veldhuis; VA Valova; AP Gaeth; N Palstra; KM Hannan; BJ Michell; LE Kelly; I Jennings; BE Kemp; RB Pearson; PJ Robinson; J Camakaris

Journal article

Phosphorylation regulates copper-responsive trafficking of the Menkes copper transporting P-type ATPase

NA Veldhuis, VA Valova, AP Gaeth, N Palstra, KM Hannan, BJ Michell, LE Kelly, I Jennings, BE Kemp, RB Pearson, PJ Robinson, J Camakaris

International Journal of Biochemistry and Cell Biology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2009

DOI: 10.1016/j.biocel.2009.06.008

Abstract

The Menkes copper-translocating P-type ATPase (ATP7A) is a critical copper transport protein functioning in systemic copper absorption and supply of copper to cuproenzymes in the secretory pathway. Mutations in ATP7A can lead to the usually lethal Menkes disease. ATP7A function is regulated by copper-responsive trafficking between the trans-Golgi Network and the plasma membrane. We have previously reported basal and copper-responsive kinase phosphorylation of ATP7A but the specific phosphorylation sites had not been identified. As copper stimulates both trafficking and phosphorylation of ATP7A we aimed to identify all the specific phosphosites and to determine whether trafficking and phospho..

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University of Melbourne Researchers

Belinda Michell Author

Richard Pearson Author

Philip Robinson Author

Grants

Funding Acknowledgements

The authors were supported by NHMRC project grants. The J.C. Rowden White Trust provided a grant for partial funding of the Olympus FV1000 confocal fluorescence microscope. N.V. was the recipient of a Melbourne Research Scholarship. R.B.P. is supported by a NHMRC Senior Research Fellowship.