FimR and FimS: Biofilm Formation and Gene Expression in Porphyromonas gingivalis
Alvin Lo, Christine Seers, Stuart Dashper, Catherine Butler, Glenn Walker, Katrina Walsh, Deanne Catmull, Brigitte Hoffmann, Steven Cleal, Patricia Lissel, John Boyce, Eric Reynolds
JOURNAL OF BACTERIOLOGY | AMER SOC MICROBIOLOGY | Published : 2010
Porphyromonas gingivalis is a late-colonizing bacterium of the subgingival dental plaque biofilm associated with periodontitis. Two P. gingivalis genes, fimR and fimS, are predicted to encode a two-component signal transduction system comprising a response regulator (FimR) and a sensor histidine kinase (FimS). In this study, we show that fimS and fimR, although contiguous on the genome, are not part of an operon. We inactivated fimR and fimS in both the afimbriated strain W50 and the fimbriated strain ATCC 33277 and demonstrated that both mutants formed significantly less biofilm than their respective wild-type strains. Quantitative reverse transcription-real-time PCR showed that expression ..View full abstract
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Awarded by National Health and Medical Research
Awarded by Australian Dental Research Foundation
This work was funded by National Health and Medical Research grant no. 509326 and Australian Dental Research Foundation grant 44/2006.