Altered fast- and slow-twitch muscle fibre characteristics in female mice with a (S248F) knock-in mutation of the brain neuronal nicotinic acetylcholine receptor
David J Cannata, David I Finkelstein, Ilse Gantois, Yaroslav Teper, John Drago, Jan M West
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY | SPRINGER | Published : 2009
We generated a mouse line with a missense mutation (S248F) in the gene (CHRNA4) encoding the alpha4 subunit of neuronal nicotinic acetylcholine receptor (nAChR). Mutant mice demonstrate brief nicotine induced dystonia that resembles the clinical events seen in patients with the same mutation. Drug-induced dystonia is more pronounced in female mice, thus our aim was to determine if the S248F mutation changed the properties of fast- and slow-twitch muscle fibres from female mutant mice. Reverse transcriptase-PCR confirmed CHRNA4 gene expression in the brain but not skeletal muscles in normal and mutant mice. Ca(2+) and Sr(2+) force activation curves were obtained using skinned muscle fibres pr..View full abstract
This study was supported by a project grant from the National Health and Medical Research Council (NHMRC). J.D. is a NHMRC practitioner fellow. Authors would like to thank Jim Massalas (Howard Florey Institute) for his assistance with the S248F mouse strain and Prof. George Stephenson ( La Trobe University) for helpful suggestions and critically reading the manuscript.