Journal article
Distribution of relaxin-3 mRNA and immunoreactivity and RXFP3-binding sites in the brain of the macaque, macaca fascicularis
S Ma, PJ Shen, Q Sang, JL Lanciego, AL Gundlach
Annals of the New York Academy of Sciences | WILEY-BLACKWELL | Published : 2009
Abstract
This study examined the presence and distribution of relaxin-3 (RLN3) and its receptor, RXFP3, throughout the brain of Macaca fascicularis. In perfusion-fixed sections, RLN3 immunoreactivity (IR) was observed throughout the fore-, mid-, and hindbrain and was enriched in the septum, hippocampus, and interpeduncular and supramammillary nuclei. Neurons in the pontine central gray area contained RLN3-IR and RLN3 mRNA. The distribution of RXFP3 protein, mapped by autoradiography of [125I]R3/I5-binding sites, correlated with the distribution of RLN3-IR. The broad distributions of RLN3-positive neuronal axons and terminals and of RXFP3 in the brain of Macaca fascicularis are consistent with finding..
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Funding Acknowledgements
This work was supported by the National Health and Medical Research Council (NHMRC) of Australia, Johnson & Johnson Pharmaceutical Research and Development LLC (USA), and the Howard Florey Biomedical Foundation (USA). SM is an NHMRC (Australia) Biomedical Postdoctoral Fellow The authors wish to thank Ross Bathgate and Geoffrey Tregear for providing the RLN3 antisera and Steve Sutton (Johnson & Johnson Pharmaceutical Research & Development, LLC, San Diego, CA, USA) and Perkin-Elmer (Boston, MA, USA) for providing [<SUP>125</SUP>I]R3/I5.