Journal article

Oligomeric resistin impairs insulin and AICAR-stimulated glucose uptake in mouse skeletal muscle by inhibiting GLUT4 translocation

Sebastian Beck Jorgensen, Jane Honeyman, Jonathan S Oakhill, Daniel Fazakerley, Jacqueline Stoeckli, Bruce E Kemp, Gregory R Steinberg

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | AMER PHYSIOLOGICAL SOC | Published : 2009

Abstract

The hormone resistin is elevated in obesity and impairs glucose homeostasis. Here, we examined the effect of oligomerized human resistin on insulin signaling and glucose metabolism in skeletal muscle and myotubes. This was investigated by incubating mouse extensor digitorum longus (EDL) and soleus muscles and L6 myotubes with physiological concentrations of resistin and assessing insulin-stimulated glucose uptake, cellular signaling, suppressor of cytokine signaling 3 (SOCS-3) mRNA, and GLUT4 translocation. We found that resistin at a concentration of 30 ng/ml decreased insulin-stimulated glucose uptake by 30-40% in soleus muscle and myotubes, whereas in EDL muscle insulin-stimulated glucose..

View full abstract

Grants

Funding Acknowledgements

These studies were supported by grants and fellowships from the National Health and Medical Research Council, Australia (G. R. Steinberg, B. E. Kemp), and a grant from the Diabetes Australia Research Trust ( S. B. Jorgensen). S. B. Jorgensen was supported by Danish Research Council of Health and Diseases postdoctoral fellowship. G. R. Steinberg is a Canadian Research Chair in Metabolism, Obesity and Type 2 Diabetes. S. B. Jorgensen was supported by the Novartis Foundation, SSMBS, and the Swiss National Foundation.