Proliferation Deficits and Gene Expression Dysregulation in Down's Syndrome (Ts1Cje) Neural Progenitor Cells Cultured From Neurospheres
Randal X Moldrich, Luce Dauphinot, Julien Laffaire, Tania Vitalis, Yann Herault, Philip M Beart, Jean Rossier, Denis Vivien, Corinne Gehrig, Stylianos E Antonarakis, Robert Lyle, Marie-Claude Potier
JOURNAL OF NEUROSCIENCE RESEARCH | WILEY | Published : 2009
Down's syndrome neurophenotypes are characterized by mental retardation and a decreased brain volume. To identify whether deficits in proliferation could be responsible for this phenotype, neural progenitor cells were isolated from the developing E14 neocortex of Down's syndrome partial trisomy Ts1Cje mice and euploid (WT) littermates and grown as neurospheres. Ts1Cje neural progenitors proliferated at a slower rate, because of a longer cell cycle, and a greater number of cells were positive for glial fibrillary acidic protein. An increase in cell death was also noted. Gene expression profiles of neural progenitor cells from Ts1Cje and WT showed that 54% of triploid genes had expression rati..View full abstract
Contract grant sponsor: European AnEUploidy grant (to M.C.P., Y.H., S.E.A.); Contract grant sponsor: Fondation Jerome Lejeune, France.