PROTEOLYTIC ACTIVITY DURING CORTICAL DEVELOPMENT IS DISTINCT FROM THAT INVOLVED IN HYPOXIC ISCHEMIC INJURY
HS Ranasinghe, CE Williams, LJ Christophidis, MD Mitchell, M Fraser, A Scheepens
NEUROSCIENCE | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2009
Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases involved in brain development and the etiology of adult cerebral injuries. In this study, we determined the MMP-2 and 9 responses following hypoxic ischemia (HI) injury in the developing brain. First, we characterized the developmental changes of MMP activity in the rat brain from embryonic day 18 (E18) to postnatal day 120 (P120). MMP-2 activity was high from E18 to P3 and decreased with age (P< or =0.001), while MMP-9 activity was not detectable. MMP-2 immunoreactivity was closely associated with differentiating cortical plate and subplate neurons. Next, we characterized the proteolytic changes after unilateral ..View full abstract
The authors wish to thank Ms. P. Pathipati for her assistance with animal surgeries. This research was supported by National Research Centre for Growth and Development, New Zealand; and Health Research Council of New Zealand.