An osmotic induction method for externally marking saltwater fishes, Stigmatopora argus and Stigmatopora nigra, with calcein
JE Smith, PI Macreadie, SE Swearer
JOURNAL OF FISH BIOLOGY | WILEY-BLACKWELL PUBLISHING, INC | Published : 2010
Calcein marking via osmotic induction and overnight immersion was compared in saltwater fishes. Immersion in a salt solution for 10 min followed by 30 min in a 500 mg l-1 calcein solution produced the highest fluorescent mark in 40 min with no effect on survival. © 2010 The Authors. Journal compilation © 2010 The Fisheries Society of the British Isles.
We are grateful for funding through an Australian Society for Fish Biology Michael Hall Innovation Award (P. I. M.) and a Holsworth Wildlife Foundation Grant (P. I. M.). We thank J. Mann for assistance with the fluorescence microscopy and two anonymous referees for their insightful comments on the manuscript. All research was conducted under University of Melbourne Animal Ethics and Department of Primary Industries Fisheries permits.