Journal article

Temporal Expression of Antioxidants in Human Cervicovaginal Fluid Associated with Spontaneous Labor

Yujing Jan Heng, Megan Katherine Waterhouse Di Quinzio, Michael Permezel, Gregory Edward Rice, Harry Michael Georgiou



Proteomic analysis of human cervicovaginal fluid (CVF) by 2D electrophoresis revealed significant differential expression of several major antioxidant enzymes during late pregnancy and term labor. Temporal quantitative changes of total antioxidant capacity (TAC), Cu,Zn superoxide dismutase (Cu,Zn SOD) and thioredoxin-1 (Trx-1) with impending term labor were investigated, and the potential of these biomarkers as individual and multiple predictors of labor was determined. The TAC of CVF (n = 193) was 8-fold significantly lower in labor, and approximately 2-fold significantly lower at 0-7, 8-14, 15-21, and 22-28 days, compared with >or=29 days prior to labor onset (p < 0.001). The expression of..

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Awarded by National Health and Medical Research Council of Australia (NHMRC)

Funding Acknowledgements

This study was supported by a National Health and Medical Research Council of Australia (NHMRC) development grant (454451) and the Medical Research Foundation for Women and Babies. YJH is a recipient of the NHMRC Biomedical (Dora Lush) postgraduate research scholarship (454880). The authors thank the Mercy Hospital for Women medical and midwifery staff for obtaining study samples. Statistical advice was provided by the Statistical Consulting Center, University of Melbourne. Microbiological assessments were performed by the Department of Microbiology, St Vincent's Hospital, Melbourne, and the Microbiology Department, Austin Pathology, Austin Health, Heidelberg, Victoria, Australia.