Journal article

Cutting Edge: Priming of CD8 T Cell Immunity to Herpes Simplex Virus Type 1 Requires Cognate TLR3 Expression In Vivo

Gayle M Davey, Magdalena Wojtasiak, Anna I Proietto, Francis R Carbone, William R Heath, Sammy Bedoui

JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2010

Abstract

Despite its potential for involvement in viral immunity, little evidence links TLR3 to adaptive antiviral responses. Here we show that TLR3 is required for the generation of CD8 T cell immunity to HSV-1. The magnitude of the gB-specific CD8 T cell response after flank infection by HSV-1 was significantly reduced in mice lacking TIR domain-containing adaptor-inducing IFN-beta or TLR3, but not MyD88. Impaired CTL induction was evident in chimeric mice lacking TLR3 in bone marrow (BM)-derived cells. Among the dendritic cell subsets, TLR3 was expressed by CD8alpha(+) dendritic cells, known to be involved in priming HSV-1-specific CD8 T cells. Use of mixed BM chimeras revealed that TLR3 and the M..

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Grants

Awarded by Deutsche Forschungsgemeinschaft


Funding Acknowledgements

This work was supported by the Deutsche Forschungsgemeinschaft (BE 3285-1/2 to S. B.), the National Health and Medical Research Council of Australia (to G.M.D., F.R.C., W.R.H., S.B.), the Australian Research Council (to F.R.C., W.R.H.), and the Howard Hughes Medical Institute (to W.R.H.). S.B. is the recipient of a National Health and Medical Research Council of Australia Career Development Award.