Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model
Kylie Hewson, Amir H Noormohammadi, Joanne M Devlin, Karim Mardani, Jagoda Ignjatovic
ARCHIVES OF VIROLOGY | SPRINGER WIEN | Published : 2009
Infectious bronchitis virus (IBV) is a coronavirus that causes upper respiratory, renal and/or reproductive diseases with high morbidity in poultry. Classification of IBV is important for implementation of vaccination strategies to control the disease in commercial poultry. Currently, the lengthy process of sequence analysis of the IBV S1 gene is considered the gold standard for IBV strain identification, with a high nucleotide identity (e.g. > or =95%) indicating related strains. However, this gene has a high propensity to mutate and/or undergo recombination, and alone it may not be reliable for strain identification. A real-time polymerase chain reaction (RT-PCR) combined with high-resolut..View full abstract
;Funding to support this research was provided by the Australian Egg Corporation Limited (AECL). The authors thank Naomi Kirkpatrick and Denise O'Rourke for technical assistance throughout this study. The authors would also like to thank Garry Anderson for help with statistical analyses.