Interpretation of urinary 8-oxo-7,8-dihydro-2 '-deoxyguanosine is adversely affected by methodological inaccuracies when using a commercial ELISA
Luke W Garratt, Vilas Mistry, Rajinder Singh, Jatinderpal Kaur Sandhu, Barbara Sheil, Marcus S Cooke, Peter D Sly
FREE RADICAL BIOLOGY AND MEDICINE | ELSEVIER SCIENCE INC | Published : 2010
The DNA lesion 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), a urinary marker of oxidative stress, is produced from reactions of reactive oxygen species with host DNA 2'-deoxyribonucleotides. The current gold-standard assessment is by complex chromatographic methods using HPLC or LC-MS/MS. Several studies have reported that commercial 8-oxodG ELISA kits correlate sufficiently with chromatographic techniques to be an easier alternative for laboratories without access to gold-standard techniques. However, the assumption that significant correlation translates into a similar ability to differentiate disease categories or treatment groups is yet to be tested. Using LC-MS/MS and two variants of ..View full abstract
This study was supported by the Australian Cystic Fibrosis Research Trust (Research grant support), Princess Margaret Hospital Foundation (Australia) (Project grant support) and University of Western Australia (Research grant support).