Effects of tissue age, presence of neurones and endothelin-3 on the ability of enteric neurone precursors to colonize recipient gut: implications for cell-based therapies
R Hotta, RB Anderson, K Kobayashi, DF Newgreen, HM Young
NEUROGASTROENTEROLOGY AND MOTILITY | WILEY | Published : 2010
BACKGROUND Most enteric neurones arise from neural crest cells that originate in the post-otic hindbrain, and migrate into and along the developing gastrointestinal tract. There is currently great interest in the possibility of cell therapy to replace diseased or absent enteric neurones in patients with enteric neuropathies, such as Hirschsprung's disease. However, it is unclear whether neural crest stem/progenitor cells will be able to colonize colon (i) in which the mesenchyme has differentiated into distinct layers, (ii) that already contains enteric neurones or (iii) that lacks a gene expressed by the gut mesenchyme, such as endothelin-3 (Et-3). METHODS Co-cultures were used to examine t..View full abstract
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Awarded by National Health and Medical Research Council of Australia
This study was supported in part by the National Health and Medical Research Council of Australia (project grants # 400017 and # 436971, and fellowships #400018 and #454773). We thank Miles Epstein for the Hu antiserum, Jeff Milbrandt and Hideki Enomoto for the RetTGM mice, and Annette Bergner and Dongcheng Zhang for technical assistance. The authors confirm that no conflict of interest exists.