Localisation of three host-protective oncospheral antigens of Taenia ovis
Abdul Jabbar, Craig T Kyngdon, Charles G Gauci, Anna K Walduck, Christina McCowan, Malcolm K Jones, Ian Beveridge, Marshall W Lightowlers
INTERNATIONAL JOURNAL FOR PARASITOLOGY | ELSEVIER SCI LTD | Published : 2010
Immunohistochemistry, confocal immunofluorescence and immunogold labelling were used to determine the localisation of the host-protective antigens To16, To18 and To45W in Taenia ovis oncospheres. During maturation of the adult tapeworm the antigens were initially seen as diffuse staining in the developing oncospheres but in mature oncospheres four distinct cells stained positively for the antigens. Confocal fluorescence microscopy using different fluorophores revealed that each of the antigens co-localises within the same cells in the oncosphere. No surface localisation was seen in non-activated or recently activated parasites. Immunogold labelling of non-activated oncosphere sections viewed..View full abstract
Related Projects (1)
Awarded by National Health and Medical Research Council
The expert technical assistance of the late Mrs. Josie Wilson, Mr. Paul Benham and Dr. Simon Crawford is greatly appreciated. We are also grateful to Mrs. Jacqueline Mills, Integrated Neuroscience Facility, Howard Florey Institute, Parkville for her assistance in co-localisation analysis. This work was supported by National Health and Medical Research Council (Grant 350279).