Journal article

Localisation of three host-protective oncospheral antigens of Taenia ovis

Abdul Jabbar, Craig T Kyngdon, Charles G Gauci, Anna K Walduck, Christina McCowan, Malcolm K Jones, Ian Beveridge, Marshall W Lightowlers

INTERNATIONAL JOURNAL FOR PARASITOLOGY | ELSEVIER SCI LTD | Published : 2010

Abstract

Immunohistochemistry, confocal immunofluorescence and immunogold labelling were used to determine the localisation of the host-protective antigens To16, To18 and To45W in Taenia ovis oncospheres. During maturation of the adult tapeworm the antigens were initially seen as diffuse staining in the developing oncospheres but in mature oncospheres four distinct cells stained positively for the antigens. Confocal fluorescence microscopy using different fluorophores revealed that each of the antigens co-localises within the same cells in the oncosphere. No surface localisation was seen in non-activated or recently activated parasites. Immunogold labelling of non-activated oncosphere sections viewed..

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Grants

Awarded by National Health and Medical Research Council


Funding Acknowledgements

The expert technical assistance of the late Mrs. Josie Wilson, Mr. Paul Benham and Dr. Simon Crawford is greatly appreciated. We are also grateful to Mrs. Jacqueline Mills, Integrated Neuroscience Facility, Howard Florey Institute, Parkville for her assistance in co-localisation analysis. This work was supported by National Health and Medical Research Council (Grant 350279).