Development and Evaluation of an ompA Quantitative Real-Time PCR Assay for Chlamydia trachomatis Serovar Determination
Matthew P Stevens, Jimmy Twin, Christopher K Fairley, Basil Donovan, Sarah E Tan, Jingxi Yu, Suzanne M Garland, Sepehr N Tabrizi
JOURNAL OF CLINICAL MICROBIOLOGY | AMER SOC MICROBIOLOGY | Published : 2010
Knowledge of circulating Chlamydia trachomatis serovars can be beneficial for sexual network surveillance, monitoring treatment success, and associating specific clinical manifestations. Typically, C. trachomatis serovars are predicted by nucleotide sequencing of four variable domains within the ompA gene. However, sequencing procedures can be labor-intensive, are not readily available, and can lack the capacity to identify multiple serovars. This study describes the development and evaluation of a quantitative real-time PCR (qPCR) test algorithm for the rapid prediction of C. trachomatis serovars, including ocular (A to C) and anogenital (D to L3) strains. This test comprises a primary qPCR..View full abstract
Awarded by Australian National Health and Medical Research Council
This study was generously supported by an Australian National Health and Medical Research Council Project grant (454620).