Pancreatic Islet Transplantation Using Vascularised Chambers Containing Nerve Growth Factor Ameliorates Hyperglycaemia in Diabetic Mice
AJ Hussey, M Winardi, J Wilson, N Forster, WA Morrison, AJ Penington, KR Knight, SJ Feeney
CELLS TISSUES ORGANS | KARGER | Published : 2010
Intraportal islet transplantation has shown initial promise for the treatment of type 1 diabetes. However, the portal vein site is associated with complications such as thrombosis and hepatic steatosis, leading to transplant failure. The aims of this study were to (1) test the feasibility of an alternative islet transplantation method that utilises a FDA-approved gelatin sponge as a novel islet carrier and (2) assess if exogenous addition of nerve growth factor (NGF) has any additional beneficial effects on graft performance in diabetic mice. Mice were rendered diabetic by a single intraperitoneal injection of streptozotocin. Five hundred syngeneic islets were seeded onto a Gelitaspon((R)) d..View full abstract
The authors gratefully acknowledge surgical assistance from Sue McKay, Liliana Pepe, Anna Deftereos and Amanda Rixon, as well as histological advice and assistance from Jason Palmer and critical review of the manuscript by Jorge Gonez. This project was supported by a grant from the NHMRC Australia.