Journal article
The multi-copper-ion oxidase CueO of Salmonella enterica serovar typhimurium is required for systemic virulence
MES Achard, JJ Tree, JA Holden, KR Simpfendorfer, OLC Wijburg, RA Strugnell, MA Schembri, MJ Sweet, MP Jennings, AG McEwan
Infection and Immunity | Published : 2010
DOI: 10.1128/IAI.01208-09
Abstract
Salmonella enterica serovar Typhimurium possesses a multi-copper-ion oxidase (multicopper oxidase), CueO (also known as CuiD), a periplasmic enzyme known to be required for resistance to copper ions. CueO from S. Typhimurium was expressed as a recombinant protein in Escherichia coli, and the purified protein exhibited a high cuprous oxidase activity. We have characterized an S. Typhimurium cueO mutant and confirmed that it is more sensitive to copper ions. Using a murine model of infection, it was observed that the cueO mutant was significantly attenuated, as indicated by reduced recovery of bacteria from liver and spleen, although there was no significant difference in recovery from Peyer's..
View full abstractGrants
Awarded by National Health and Medical Research Council of Australia
Awarded by Australian Postgraduate
Funding Acknowledgements
This work was supported by Program Grant 284214 from the National Health and Medical Research Council of Australia to A. G. M., M.P.J., and R. A. S. and Project Grant 519722 to A. G. M. and M. A. S. J.J.T. was the recipient of an Australian Postgraduate