Journal article

Characterisation of Nitric Oxide Synthase in Three Cnidarian-Dinoflagellate Symbioses

Helena Safavi-Hemami, Neil D Young, Jason Doyle, Lyndon Llewellyn, Anke Klueter

PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2010

Abstract

BACKGROUND: Nitric oxide synthase (NOS) is an enzyme catalysing the conversion of L-arginine to L-citrulline and nitric oxide (NO), the latter being an essential messenger molecule for a range of biological processes. Whilst its role in higher vertebrates is well understood little is known about the role of this enzyme in early metazoan groups. For instance, NOS-mediated signalling has been associated with Cnidaria-algal symbioses, however controversy remains about the contribution of enzyme activities by the individual partners of these mutualistic relationships. METHODOLOGY/PRINCIPAL FINDINGS: Using a modified citrulline assay we successfully measured NOS activity in three cnidarian-algal ..

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Funding Acknowledgements

This research was funded by the Cooperative Research Centre for the Great Barrier Reef World Heritage Area (CRC Reef Research). URL: http//www.rrrc.org.au/crcreef/index.html. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.