Insulin, a key regulator of hormone responsive milk protein synthesis during lactogenesis in murine mammary explants
Karensa K Menzies, Heather J Lee, Christophe Lefevre, Christopher J Ormandy, Keith L Macmillan, Kevin R Nicholas
FUNCTIONAL & INTEGRATIVE GENOMICS | SPRINGER HEIDELBERG | Published : 2010
Murine milk protein gene expression requires insulin, hydrocortisone, and prolactin; however, the role of insulin is not well understood. This study, therefore, examined the requirement of insulin for milk protein synthesis. Mammary explants were cultured in various combinations of the lactogenic hormones and global changes in gene expression analysed using Affymetrix microarray. The expression of 164 genes was responsive to insulin, and 18 were involved in protein synthesis at the level of transcription and posttranscription, as well as amino acid uptake and metabolism. The folate receptor gene was increased by fivefold, highlighting a potentially important role for the hormone in folate me..View full abstract
This work was supported by Dairy Australia and the Co-operative Research Centre for Innovative Dairy Products.