Journal article

Structural and functional characterisation of the fork head transcription factor-encoding gene, Hc-daf-16, from the parasitic nematode Haemonchus contortus (Strongylida)

Min Hu, James B Lok, Najju Ranjit, Holman C Massey, Paul W Sternberg, Robin B Gasser

INTERNATIONAL JOURNAL FOR PARASITOLOGY | ELSEVIER SCI LTD | Published : 2010

Abstract

Despite their phylogenetic diversity, parasitic nematodes share attributes of longevity and developmental arrest (=hypobiosis) with free-living nematodes at key points in their life cycles, particularly in larval stages responsible for establishing infection in the host. Insulin-like signalling plays crucial roles in the regulation of life span and arrest (=dauer formation) in the free-living nematode, Caenorhabditis elegans. Insulin-like signalling in C. elegans negatively regulates the fork head boxO (FoxO) transcription factor encoded by daf-16, which is linked to initiating a dauer-specific pattern of gene expression. Orthologues of daf-16 have been identified in several species of paras..

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Grants

Awarded by Australian Research Council (ARC)


Awarded by National Institutes of Health


Awarded by Ellison Medical Foundation


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work was supported by grants from the Australian Research Council (ARC) (LP0667795 and LX0882231), Genetic Technologies Limited, Meat and Livestock Australia, the Australian Academy of Science, the Australian-American Fulbright Commission (R.B.G.), the National Institutes of Health (AI-50688 to J.B.L.) and The Ellison Medical Foundation (ID-IA-0037-02 to J.B.L.). The authors thank Ben Datu for sharing primers DAF-16F100 and DAF-16R100. The C. elegans strains used in this study were originally provided by the Caenorhabditis Genetics Center, University of Minnesota, which is funded by the NIH National Center for Research Resources.