Transcriptional analysis of the grlRA virulence operon from Citrobacter rodentium

M Tauschek; J Yang; D Hocking; K Azzopardi; A Tan; E Hart; J Praszkier; RM Robins-Browne

Journal article

Transcriptional analysis of the grlRA virulence operon from Citrobacter rodentium

M Tauschek, J Yang, D Hocking, K Azzopardi, A Tan, E Hart, J Praszkier, RM Robins-Browne

Journal of Bacteriology | Published : 2010

DOI: 10.1128/JB.01540-09

Abstract

The locus for enterocyte effacement (LEE) is the virulence hallmark of the attaching-and-effacing (A/E) intestinal pathogens, namely, enteropathogenic Escherichia coli, enterohemorrhagic E. coli, and Citrobacter rodentium. The LEE carries more than 40 genes that are arranged in several operons, e.g., LEE1 to LEE5. Expression of the various transcriptional units is subject to xenogeneic silencing by the histone-like protein H-NS. The LEE1-encoded regulator, Ler, plays a key role in relieving this repression at several major LEE promoters, including LEE2 to LEE5. To achieve appropriate intracellular concentrations of Ler in different environments, A/E pathogens have evolved a sophisticated reg..

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University of Melbourne Researchers

Roy Robins-Browne Author

Aimee Tan Author

Related Projects (1)

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Grants

Funding Acknowledgements

Work in our laboratory is supported by research grants from the Australian National Health and Medical Research Council and the Australian Research Council. M. Tauschek is supported by a Peter Doherty Fellowship of the Australian National Health and Medical Research Council, D. Hocking is the recipient of a Melbourne Research Scholarship, and A. Tan is the recipient of an Australian Postgraduate Award.