Elucidating the identity of Anisakis larvae from a broad range of marine fishes from the Yellow Sea, China, using a combined electrophoretic-sequencing approach
Chunxia Du, Luping Zhang, Meiqing Shi, Zhu Ming, Min Hu, Robin B Gasser
ELECTROPHORESIS | WILEY | Published : 2010
Larval nematodes were collected from marine fishes from the Yellow Sea, China. Specimens (n=1731) of Anisakis type I from 311 fishes (representing 40 species) were each identified based on morphological characters. From the genomic DNA from individual specimens, a region of nuclear ribosomal DNA was amplified by PCR, followed by digestion with restriction endonuclease HinfI, TaqI or HhaI. Subsequently, the ITS-1 and ITS-2 regions of selected samples were sequenced. The results revealed three species of Anisakis, namely Anisakis pegreffii (n=1709), A. typica (n=3) and a genotype (n=19) proposed, also based on comparison with previous studies, to be a "hybrid" between A. pegreffii and A. simpl..View full abstract
Awarded by Department of Education, People s Republic China
Thanks to Liang Li, Institute of Zoology, Chinese Academy of Sciences, and Zhen Xu, College of Medical Science, Hebei Engineering University for the collection of specimens. This study was supported by a grant (No. 20070094005) from Department of Education, People s Republic China to LZ.