Journal article

In vivo tracking of 'color-coded' effector, natural and induced regulatory T cells in the allograft response

Zhigang Fan, Joel A Spencer, Yan Lu, Costas M Pitsillides, Gurbakhshish Singh, Pilhan Kim, Seok H Yun, Vasilis Toxavidis, Terry B Strom, Charles P Lin, Maria Koulmanda

NATURE MEDICINE | NATURE PUBLISHING GROUP | Published : 2010

Abstract

Here we present methods to longitudinally track islet allograft-infiltrating T cells in live mice by endoscopic confocal microscopy and to analyze circulating T cells by in vivo flow cytometry. We developed a new reporter mouse whose T cell subsets express distinct, 'color-coded' proteins enabling in vivo detection and identification of effector T cells (T(eff) cells) and discrimination between natural and induced regulatory T cells (nT(reg) and iT(reg) cells). Using these tools, we observed marked differences in the T cell response in recipients receiving tolerance-inducing therapy (CD154-specific monoclonal antibody plus rapamycin) compared to untreated controls. These results establish re..

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University of Melbourne Researchers

Grants

Awarded by US National Institutes of Health


Awarded by Juvenile Diabetes Research Foundation (JDRF)


Awarded by Medical Research Council


Awarded by NATIONAL EYE INSTITUTE


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work was supported by grants from the US National Institutes of Health (R21 AI081010 to S.H.Y. and R01 EY14106 to C.P.L. and P01-AI041521 and P01-AI041521-S1 to T.B.S.) and the Juvenile Diabetes Research Foundation (JDRF 7-2005-1329 to T.B.S. and M.K.).