Journal article

Establishment of the 1st World Health Organization international standards for human papillomavirus Type 16 DNA and Type 18 DNA

Dianna E Wilkinson, Sally A Baylis, David Padley, Alan B Heath, Morag Ferguson, Sonia R Pagliusi, Wim G Quint, Cosette M Wheeler

INTERNATIONAL JOURNAL OF CANCER | WILEY | Published : 2010

Abstract

A World Health Organization collaborative study was conducted to evaluate candidate international standards for human papillomavirus (HPV) Type 16 DNA (NIBSC code 06/202) and HPV Type 18 DNA (NIBSC code 06/206) for use in the amplification and detection steps of nucleic acid-based assays. The freeze-dried candidate international standards were prepared from bulk preparations of cloned plasmid containing full-length HPV-16 or HPV-18 genomic DNA. Nineteen laboratories from 13 countries participated in the study using a variety of commercial and in-house quantitative and qualitative assays. The data presented here indicate that, upon freeze-drying, there is no significant loss in potency for th..

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