Massively Parallel Sequencing and Analysis of the Necator americanus Transcriptome
Cinzia Cantacessi, Makedonka Mitreva, Aaron R Jex, Neil D Young, Bronwyn E Campbell, Ross S Hall, Maria A Doyle, Stuart A Ralph, Elida M Rabelo, Shoba Ranganathan, Paul W Sternberg, Alex Loukas, Robin B Gasser
PLOS NEGLECTED TROPICAL DISEASES | PUBLIC LIBRARY SCIENCE | Published : 2010
BACKGROUND: The blood-feeding hookworm Necator americanus infects hundreds of millions of people worldwide. In order to elucidate fundamental molecular biological aspects of this hookworm, the transcriptome of the adult stage of Necator americanus was explored using next-generation sequencing and bioinformatic analyses. METHODOLOGY/PRINCIPAL FINDINGS: A total of 19,997 contigs were assembled from the sequence data; 6,771 of these contigs had known orthologues in the free-living nematode Caenorhabditis elegans, and most of them encoded proteins with WD40 repeats (10.6%), proteinase inhibitors (7.8%) or calcium-binding EF-hand proteins (6.7%). Bioinformatic analyses inferred that the C. elegan..View full abstract
This research was supported by grants from the National Human Genome Research Institute (NHGRI) and National Institutes of Health (MM) and the Australian Research Council, the Australian Academy of Science and the Australian-American Fulbright Commission (RBG). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.