Highly sensitive non-isotopic restriction endonuclease fingerprinting of nucleotide variability in the gp60 gene within Cryptosporidium species, genotypes and subgenotypes infective to humans, and its implications
Aradhana Pangasa, Aaron R Jex, Matthew J Nolan, Bronwyn E Campbell, Shane R Haydon, Melita A Stevens, Robin B Gasser
ELECTROPHORESIS | WILEY | Published : 2010
The high-resolution analysis of genetic variation has major implications for the identification of parasites and micro-organisms to species and subspecies as well as for population genetic and epidemiological studies. In this study, we critically assessed the effectiveness of a PCR-based restriction endonuclease fingerprinting (REF) method for the detection of mutations in the 60 kDa glycoprotein gene (gp60) of Cryptosporidium, a genus of parasitic protists of major human and animal health importance globally. This gene displays substantial intraspecific variability in sequence, particularly in a TCA (perfect and imperfect) microsatellite region, is present as a single copy in the nuclear ge..View full abstract
Financial support was provided by Melbourne Water Corporation, the Australian Research Council (R. B. G. and A. R. J.) and the National Health and Medical Research Council (A. R. J.). The authors are grateful to Margaret Whipp for providing some samples used in this study.