Biodistribution of canine leucocytes labelled with technetium-99m stannous fluoride colloid in whole blood and their ability to localise to sites of induced inflammation

Abstract

This study assessed the biodistribution of autologous leucocytes radiolabelled with technetium-99m stannous fluoride colloid (99mTcSnC) for detection of foci of induced inflammation in dogs. Venous blood was collected from seven healthy dogs and incubated with 99mTcSnC for 1h at room temperature. Radiolabelled samples were injected intravenously (IV) and the dogs were scanned using a gamma camera. Another seven healthy dogs were injected intradermally with tumour necrosis factor α and then IV with 99mTcSnC radiolabelled autologous blood 3h later before being scanned. The radiolabelled leucocytes localised to sites of inflammation by 30min post-injection. IV injection of autologous leucocytes..