Journal article

A Serial Analysis of Gene Expression Profile of the Alzheimer's Disease Tg2576 Mouse Model

Amee J George, Lavinia Gordon, Tim Beissbarth, Irene Koukoulas, RM Damian Holsinger, Victoria Perreau, Roberto Cappai, Seong-Seng Tan, Colin L Masters, Hamish S Scott, Qiao-Xin Li

NEUROTOXICITY RESEARCH | SPRINGER | Published : 2010

Abstract

Serial analysis of gene expression (SAGE), a technique that allows for the simultaneous detection of expression levels of the entire genome without a priori knowledge of gene sequences, was used to examine the transcriptional expression pattern of the Tg2576 mouse model of Alzheimer's disease (AD). Pairwise comparison between the Tg2576 and nontransgenic SAGE libraries identified a number of differentially expressed genes in the Tg2576 SAGE library, some of which were not previously revealed by the microarray studies. Real-time PCR was used to validate a panel of genes selected from the SAGE analysis in the Tg2576 mouse brain, as well as the hippocampus and temporal cortex of sporadic AD and..

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Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by NIH/NIA


Awarded by NATIONAL INSTITUTE ON AGING


Funding Acknowledgements

This work was supported in part by grants from the National Health and Medical Research Council of Australia #208978 (AG, RH, RC, QXL, CM). AG was a recipient of Alzheimer's Australia Research travel grant. Funding for the UCI-ADRC was provided by NIH/NIA grant P50 AG16573. We would also like to thank Karen Hsiao-Ashe for the Tg2576 mice.