Journal article

A Customized Gene Expression Microarray Reveals That the Brittle Stem Phenotype fs2 of Barley Is Attributable to a Retroelement in the HvCesA4 Cellulose Synthase Gene

Rachel A Burton, Gang Ma, Ute Baumann, Andrew J Harvey, Neil J Shirley, Jillian Taylor, Filomena Pettolino, Antony Bacic, Mary Beatty, Carl R Simmons, Kanwarpal S Dhugga, J Antoni Rafalski, Scott V Tingey, Geoffrey B Fincher



The barley (Hordeum vulgare) brittle stem mutants, fs2, designated X054 and M245, have reduced levels of crystalline cellulose compared with their parental lines Ohichi and Shiroseto. A custom-designed microarray, based on long oligonucleotide technology and including genes involved in cell wall metabolism, revealed that transcript levels of very few genes were altered in the elongation zone of stem internodes, but these included a marked decrease in mRNA for the HvCesA4 cellulose synthase gene of both mutants. In contrast, the abundance of several hundred transcripts changed in the upper, maturation zones of stem internodes, which presumably reflected pleiotropic responses to a weakened cel..

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University of Melbourne Researchers