Differences in transcription between free-living and CO2-activated third-stage larvae of Haemonchus contortus
Cinzia Cantacessi, Bronwyn E Campbell, Neil D Young, Aaron R Jex, Ross S Hall, Paul JA Presidente, Jodi L Zawadzki, Weiwei Zhong, Boanerges Aleman-Meza, Alex Loukas, Paul W Sternberg, Robin B Gasser
BMC GENOMICS | BMC | Published : 2010
BACKGROUND: The disease caused by Haemonchus contortus, a blood-feeding nematode of small ruminants, is of major economic importance worldwide. The infective third-stage larva (L3) of this gastric nematode is enclosed in a cuticle (sheath) and, once ingested with herbage by the host, undergoes an exsheathment process that marks the transition from the free-living (L3) to the parasitic (xL3) stage. This study explored changes in gene transcription associated with this transition and predicted, based on comparative analysis, functional roles for key transcripts in the metabolic pathways linked to larval development. RESULTS: Totals of 101,305 (L3) and 105,553 (xL3) expressed sequence tags (EST..View full abstract
Awarded by University of Melbourne
This work was supported by grants from the Australian Research Council (RBG, AL, PWS) and the Australian-American Fulbright Commission (RBG). CC is the grateful recipient of International Postgraduate Research Scholarship (IPRS) from the Australian Government and a fee-remission scholarship through the University of Melbourne as well as the Clunies Ross (2008) and Sue Newton (2009) awards from the School of Veterinary Science of the same university. The staff of WormBase is acknowledged for their contributions. This article is dedicated to the late Paul JA Presidente.