alpha-Synuclein induced membrane depolarization and loss of phosphorylation capacity of isolated rat brain mitochondria: Implications in Parkinson's disease
Kalpita Banerjee, Maitrayee Sinha, Chi Le Lan Pham, Sirsendu Jana, Dalia Chanda, Roberto Cappai, Sasanka Chakrabarti
FEBS Letters | WILEY | Published : 2010
This study demonstrates that in vitro incubation of isolated rat brain mitochondria with recombinant human alpha-synuclein leads to dose-dependent loss of mitochondrial transmembrane potential and phosphorylation capacity. However, alpha-synuclein does not seem to have any significant effect on the activities of respiratory chain complexes under similar conditions of incubation suggesting that the former may impair mitochondrial bioenergetics by direct effect on mitochondrial membranes. Moreover, the recombinant wild type alpha-synuclein and different mutant forms (A30P, A53T and E46K) have essentially similar effects on rat brain isolated mitochondria. The results are significant in view of..View full abstract
Awarded by Life Sciences Research Board, Defence Research and Development Organization, Government of India
The work was supported by Life Sciences Research Board, Defence Research and Development Organization, Government of India (No. LSRB-93/EPB/2006). This work was also supported by funding from the National Health and Medical Research Council (NHMRC) of Australia to RC. RC is an NHMRC Senior Research Fellow. KB was supported by an award of Senior Research Fellowship from Indian Council of Medical Research, New Delhi.