Journal article

Homeobox gene distal-less 3 is expressed in proliferating and differentiating cells of the human placenta

A Chui, NA Pathirage, B Johnson, M Cocquebert, T Fournier, D Evain-Brione, B Roald, U Manuelpillai, SP Brennecke, B Kalionis, P Murthi

PLACENTA | W B SAUNDERS CO LTD | Published : 2010


DLX3, a member of the large homeobox gene family of transcription factors, is necessary for normal placentation. Targeted deletion of dlx3 in mouse resulted in embryonic death due to placental failure. This study demonstrates the presence of DLX3 mRNA expression in human first trimester and term placental tissue, cultured trophoblast-like cell lines and in isolated primary villous and extravillous trophoblast cells. Using an ovine polyclonal antibody, the spatial distribution was identified for DLX3 in human placental tissues, trophoblast cell lines and in freshly isolated primary trophoblast cells. A 50 kDa immunoreactive DLX3 protein was detected in the human placenta, in trophoblast cell ..

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Awarded by Australian National Health and Medical Research Council (NHMRC)

Funding Acknowledgements

The authors wish to thank the consenting patients and the clinical and research midwives, Sue Nisbet and Sue Duggan, for the supply of term-control placental tissues. Funding support for this work was provided from the Australian National Health and Medical Research Council (NHMRC project grant #509140) to Dr. P. Murthi, and by the postgraduate scholarship support to A. Chui from The Royal Women's Hospital and an Arthur Nylasy Award and a Felix Meyer Postgraduate Scholarship, both from The University of Melbourne.