Journal article
Extracellular proteomes of M-CSF (CSF-1) and GM-CSF-dependent macrophages
MJ Bailey, DC Lacey, BVA De Kok, PD Veith, EC Reynolds, JA Hamilton
Immunology and Cell Biology | WILEY | Published : 2011
DOI: 10.1038/icb.2010.92
Abstract
Macrophage colony-stimulating factor (M-CSF) (also known as CSF-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF) have distinct effects on macrophage lineage populations, which are likely to be contributing to their functional heterogeneity. A comparative proteomic analysis of proteins released into culture media from such populations after M-CSF and GM-CSF exposure was carried out. Adherent macrophage populations, termed bone marrow-derived macrophage (BMM) and GM-BMM, were generated after treatment of murine bone marrow precursors with M-CSF and GM-CSF, respectively. Proteins in 16-h serum-free conditioned media (CM) were identified by two-dimensional gel electrophoresis and..
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Awarded by Australian NHMRC
Funding Acknowledgements
This work was funded in part by the Cooperative Research Centre (CRC) for Chronic Inflammatory Diseases, and grants from the National Health and Medical Research Council (NHMRC) to JAH and from the Helen Macpherson Smith Trust (JAH/MJB). JAH is also supported by a NHMRC Senior Principal Research Fellowship. DCL was also supported by AFA-ARA Heald Fellowship from the Arthritis Foundation of Australia. Proteomic data analysis described in this work was supported by the use of the Australian Proteomics Computational Facility funded by the Australian NH&MRC under grant No. 381413.